Valoración de tres dilutores en la criopreservación de semen de gallos de riña

Abstract

Three dilutors were evaluated in the preservation of semen from kidney cocks, applying assisted reproduction techniques. This research was carried out in the Animal Reproduction Laboratory of the Faculty of Animal Sciences belonging to ESPOCH, located in the province of Chimborazo, Riobamba canton. With an experimental methodology, under a Completely Random Design, using 3 treatments with 6 repetitions, the experimental unit consisted of 1 rooster, with a duration of 90 days. The evaluation of post-defrosting mass motility of seminal doses reported highly significant differences between treatment means at (P <0.01), registering a higher value for the 1 +10% glycerol dilutor with 51.83% unlike the 2 + 15% glycerol dilutor with which the lowest percentage was obtained by defrosting the straws with 10.33%, for the motility variable individual reporting the highest value with the 1 + 10% glycerol dilutor, obtaining 3 points, unlike the lower value which was registered for the 3 Andromed dilutor with 0.58 points, in terms of sperm viability, highly significant differences were recorded between treatment averages (P <0.01). reporting the greatest sperm viability when using the dilutor 1-10% glycerol with 47.5%. unlike the lower value which was registered with the commercial dilutor Andromed with 13.33%, which allows us to deduce that the diluents used in the process of semen cryopreservation in kidney cocks influenced seminal quality. Concluding that the best microscopy characteristics were presented when using dilutor 1. with a mass motility of 51.83%, individual 3pts, and a sperm viability of 47.5%, recommending using this research as a basis for seminal conservation in birds, especially in endangered species.