Determinación del potencial inhibitorio de la fracción alcaloidal de Psychotria poeppigiana sobre xantina oxidasa y radical superóxido mediante autografía.

Abstract

The present study aimed to determine the inhibitory potential of the alkaloidal fraction of Psychotria poeppigiana on xanthine oxidase and superoxide radical by autography. For this target, the xanthine substrate was used, the alkaloidal fraction at different concentrations (1, 5, 10, 50, 100 µg) was dissolved in a mixture with phosphate buffer solution and dimethylsulfoxide and xanthine oxidase. The ability to capture superoxide radical was determined using two techniques; the first was in charge of the system: Riboflavin, tetrazolium Nitroazul and light; in the second, the system formed by NADH, tetrazolium nitroazul and phenazine methosulfate, the standard present in both cases was taxifoline. Thin layer chromatography (TLC) plates were used, the samples were seeded using the dot-blot technique, the mobile phase was composed of chloroform, triethylamine and methanol (6: 0, 5: 0, 6), and wavelength to reveal the plates was 254nm. The standard that was used to assess the antioxidant activity using the DPPH method was ascorbic acid, meanwhile, to evaluate the inhibition potential on xanthine oxidase the standard was Alopurinol and the xanthine substrate. From the tests performed, it was identified a superoxide radical scavenger and a pure xanthine oxidase inhibitor. The GelAnalyzer software determined the areas of inhibition. The data obtained both quantitative and qualitative can be reflected in the diameter of the halos formed and in the color of the solutions respectively. The alkaloidal extract of Psychotria poeppigiana has the capacity to capture superoxid radicals against the Riboflavin/Light/NBT and NADH/PMS/NBT systems, while the inhibition on xanthine oxidase is void. Further studies should be carried out on the extract of Psychotria poeppigianam since it presents an extensive number of compounds with pharmacological activities.