Identificación molecular de bacterias electrogénicas a partir de la biopelícula anódica de celdas de combustible microbiana para la producción de bioelectricidad.

Abstract

This degree work's main aim was to perform the molecular identification of electrogenic bacteria from the anodic biofilm of microbial fuel cells that were developed from soil samples coming from the Andeans high lands of Chimborazo-Ecuador, for the study of bioelectricity production. The microbial isolation of two anodic biofilms extracted from reactivated cells was performed, by sowing through extension in enriched solid médium surface and subsequent incubation at 22.5 ° C; From the morphological characterization of the colonies obtained, thirteen pure cultures of the most representative microbial types were obtained for their molecular identification. To develop the bioelectricity production tests, the cells were reactivated by selecting three of the eight bacteria identified, based on compliance with the following characteristics: non-pathogenic, facultative anaerobic, degrading and good bioclectricity producers, finally choosing the strains Serratia sp., Rhodococcuserythropolis and Citrobacterfreundii. The voltage level was measured three times a day for 15 days, and the results show an approximate generation of 0.3 to 0.5 V at slightly acidic ph. The bacterial strains identified were stored in glycerol at 4 ° C for further research. It is suggested to propose bioelectricity generation tests from bacterial consortiums formulated with the isolated strains.