Evaluación de la actividad inhibitoria del extracto de alcaloides de Phaedranassa viridiflora sobre acetilcolinesterasa y butirilcolinesterasa.

Abstract

The general objective was to determine the inhibitory activity of the alkaloid extract on acetylcholinesterase and butyrylcolinesterase. The Amaryllidaceae family is extensively studied for its alkaloids, which have important physiological activities, including their use in the treatment of Alzheimer’s disease. Given the few studies on the endemic species Phaedranassa viridiflora. For this, the extraction of bulb alkaloids of the varieties of Phaedranassa viridiflora was carried out, using organic solvents and pH changes. The identification of its alkaloids was carried out by gas chromatography analysis coupled to mass spectrometry (CG-MS). The method of Ellman et al., (1996), with some modifications by Ortiz et al. (2016) was used to verify its inhibition activity against the enzyme acetylcholinesterase and butyrylcholinesterase using galanthamine compound as a reference marker. In the P.viridiflora species, yellow flower, haemantamine homolicorinaand tazetina alkaloids were identified, while in the orange flower of P.viridiflora there alkaloids such as haemantamine, tazetina, galantamine, lycorine, incartina an galantine. The results of the orange variety of Phaedranassa viridiflora showed a very high inhibitory activity on AChE presenting an IC50 value= 1,02± 0,05ug/mL, like the galantamine control (IC50 = 0,57± 0,07 ug/mL). the inhibitory activity on BuChE was moderately high, presenting an IC50 value of 84,28 ± 4,28 ug/mL, and the galantamine control showed IC values of 13,53 ± 1,28 ug/mL. in the case of the yellow variety of Phaedranassa viridiflora, it showed a high inhibitory activity on AChE(7,82± 0,13ug/mL) and low activity on BuChE (125,51 ± 3,03 ug/mL). Therefore, the orange variety of Phaedranassa viridiflora, was the one with the best activity. Therefore, both varieties could be considered as objtetive in the search for new cholinesterase inhibitors.