Evaluación del efecto antiinflamatorio de Ilex guayusa (Loes), Vernonanthura patens (Kunth) y Theobroma cacao (Linneo) en el modelo Animal Rata (Rattus norvegicus) de laboratorio.

Abstract

The objective of the present titration work was to verify the anti-inflammatory activity of the extracts of the leaves of Ilex guayusa, Vernonanthura patens and Theobroma cacao, applying two doses. This work was carried out in collaboration with the Center for Biotechnological Research of Ecuador, ESPOL. Control of the quality of the drug phenolic type, flavonoids, alkaloids saponins. The content of the total flavonoids and alkaloid phenols was determined by spectrophotometric techniques. The evolution of the anti-inflammatory effect was based on the method subplantar edema induced by carrageenan at 15, under the plantar aponeurosis of the left leg of the rat. Twenty-four rats were used, which were randomly selected and divided into 8 groups of 3 rats. It was used as a positive control (d iclofenac sodium) and negative control (distilled water), extracts of Ilex guayusa, Vernonanthura patens and Theobroma cacao concentration of 100 mg/kg and 300 mg/kg. The administration of the treatments was orally 30 minutes before the induction of the pathology. The paw volume was measured and the inhibition percentage of inflammation was calculated by means of the program program Microsoft office Excel 2013. For the statistical analysis, the univariate and DUNCAN test were applied. The extracts of Ilex guayusa and Theobroma cacao (300 mg/kg) had a greater anti-inflammatory effect in 24 hours after the induction of the pathology, and Vernonanthura patens (100 mg/kg) presented a lower percentage of inhibition, there is no difference significant difference between T. cacao at 300 mg /kg and the positive control. It is recommended to carry out toxicological studies of the three species.