Determinación de la actividad ansiolítica del extracto hidroalcohólico de las flores de lavanda (Lavandula officinalis) en ratones (Mus musculus).

Abstract

The objective of this research work was to determine the anxiolytic activity of the Lavandula officinalis flowers hydroalcoholic extract in mice (Mus musculus). The vegetable matter was collected, dried, crushed and macerated with 70% ethanol in a 1:10 ratio for three days with constant agitations, through phytochemical screening, secondary metabolites were recognized qualitatively, such as alkaloids, terpenes, spheroids, phenolic compounds and flavonoids of great interest. Subsequently, the extract was concentrated until the alcohol was eliminated until it was completely dry. To evaluate the anxiolytic activity, 30 mice were used, weighing 25-30 g and with an age of 2 - 3 months, they were grouped according to the treatments administered. 15% propylene glycol was used as a negative control and the extracts at concentrations of 25, 100 and 200 mg/kg, and Clonazepam as a positive control. Exploratory tests were done with the 3 teams: Dark-light room, Open field and Elevated cross labyrinth. Data were captured on videos, which were then processed using software: ITOPI for “Dark-light room" and "Elevated cross labyrinth" and EMIS for "Open field". Data obtained were statistically analyzed, stating that the dose of 200mg/kg presented anxiolytic activity in the two most relevant teams. In addition to the sedative activity, they do not affect motor activity. It is recommended to carry out further studies to verify the anxiolytic activity of Lavandula officinalis with higher doses than those used to confirm its effect.